FAQs about Specimen Freezing, Storing & Shipping
How do I keep ice cream frozen with dry ice for
Today, it is theoretically assumed that frozen storage of semen doses in liquid nitrogen guarantees sperm functionality indefinitely. Sperm viability and full sperm motility were analyzed by CASA system, and acrosome integrity was assessed with Coomassie blue staining. However, future field trials will be necessary in order to validate that both fertility and embryo viability are maintained for the times analyzed. Cryopreservation in Eukaryotes. Cryopreservation protocols for the bull used in the animal production industry began in the s [ 1 ]. Since then, both the packaging type and cryopreservation system were changing on the time.
Cryopreserved specimens, when properly stored, will remain viable for an indefinite amount of time. Pregnancies have been reported for cases in which the sperm has been stored for 20 years prior to thawing and insemination. All of our specimens are shipped in liquid nitrogen vapor tanks via FedEx, local courier, or local patient pick up. The liquid nitrogen vapor tank is inside of a hard outer case for FedEx orders and a cardboard box for local orders.
This study aimed to test the effects on sperm viability of transporting cryopreserved semen samples on dry ice. Twenty normozoospermic semen samples were cryopreserved and divided into five groups. The samples in Group 1 were immersed in liquid nitrogen throughout the experiment in cryogenic storage tanks; the cryopreserved straws in Group 2 were placed in a Styrofoam box containing dry ice and kept under these conditions for 48 hours; the samples in Group 3 were kept for 48 hours on dry ice under the same conditions as the Group 2 samples, and were then moved to a storage tank filled with liquid nitrogen; Group 4 samples were also kept for 48 hours in dry ice storage, and the Styrofoam box containing the samples was shipped by plane to assess the effects of shipping; the samples in Group 5 were shipped together with the Group 4 samples and were placed in a storage tank with liquid nitrogen after spending 48 hours stored on dry ice. After thawing, sperm parameters were analyzed for viability, vitality, and motility; spermatozoa were also tested for mitochondrial activity.